simplechip chip seq dna library prep kit  (Cell Signaling Technology Inc)

Journal: Bladder Cancer

Article Title: Association of Histone H3 Trimethylation in Circulating Monocytes with Lack of Early Recurrence in Patients with Bladder Cancer following BCG Induction Therapy

doi: 10.3233/BLC-230028

Figure Lengend Snippet: Characteristics of patients enrolled in the ChIP-seq study

Article Snippet: Chromatin immunoprecipitation sequencing (ChIP-seq) libraries were constructed using SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina and SimpleChIP ChIP-seq Multiplex Oligos for Illumina (Cell Signaling Technology) according to the manufacturer’s instructions.

Techniques:

Journal: Bladder Cancer

Article Title: Association of Histone H3 Trimethylation in Circulating Monocytes with Lack of Early Recurrence in Patients with Bladder Cancer following BCG Induction Therapy

doi: 10.3233/BLC-230028

Figure Lengend Snippet: H3K4me3 methylation at promoters of innate immune memory-related genes. A) peripheral blood from patients with NMIBC was collected before the first (week 1) and last (week 6) intravesical BCG instillation of the first induction phase. TURBT: transurethral resection of bladder tumor; BCG: bacillus Calmette-Guérin. B) H3K4me3 in monocytes at promoters of TNF α and TLR4 genes before the first BCG instillation (week 1) and the last (week 6). Blue solid shapes: no-early recurrence (NR) patients used for ChIP-seq; red solid shapes: early recurrence (R) patients used for ChIP-seq; circles: week 1 (pre-BCG); squares: week 6 (before 6th BCG instillation).

Article Snippet: Chromatin immunoprecipitation sequencing (ChIP-seq) libraries were constructed using SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina and SimpleChIP ChIP-seq Multiplex Oligos for Illumina (Cell Signaling Technology) according to the manufacturer’s instructions.

Techniques: Methylation, ChIP-sequencing

Journal: Bladder Cancer

Article Title: Association of Histone H3 Trimethylation in Circulating Monocytes with Lack of Early Recurrence in Patients with Bladder Cancer following BCG Induction Therapy

doi: 10.3233/BLC-230028

Figure Lengend Snippet: Validation of gene promoter elements identified as enriched in patients without early recurrence. A) H3K4me3 in monocytes at the promoter sequences of FZD5 , LEPR and IRS2 . B) H3K4me3 in monocytes at promoter sequences of AKT3 and MET . Circles: week 1 (pre-BCG); squares: week 6 (before 6th BCG instillation) C) Two-dimensional t-SNE embeddings of ChIP-qPCR data at week 1 (left panel) and week 6 (right panel) obtained from monocyte samples of patients without early recurrence (NR, blue) and patients with early recurrence (R, red). Blue solid shapes: monocytes from patients with no early recurrence (NR) used for ChIP-seq; red solid shapes: monocytes from patients with early recurrence (R) used for ChIP-seq.

Article Snippet: Chromatin immunoprecipitation sequencing (ChIP-seq) libraries were constructed using SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina and SimpleChIP ChIP-seq Multiplex Oligos for Illumina (Cell Signaling Technology) according to the manufacturer’s instructions.

Techniques: ChIP-sequencing

Journal: Bladder Cancer

Article Title: Association of Histone H3 Trimethylation in Circulating Monocytes with Lack of Early Recurrence in Patients with Bladder Cancer following BCG Induction Therapy

doi: 10.3233/BLC-230028

Figure Lengend Snippet: Methylation levels on monocytes from an independent validation cohort of patients at gene promoters previously identified as enriched in the original group of patients without early disease recurrence. A) H3K4me3 in monocytes at gene promoters for FZD5 , LEPR and IRS2 genes. B) H3K4me3 in monocytes at gene promoters for AKT3 and MET genes. C) Two-dimensional t-SNE embeddings of ChIP-qPCR data from monocytes of a validation cohort at week one (left panel) and week six (right panel). Blue solid shapes: monocytes from patients with no early recurrence (NR) used for ChIP-seq; red solid shapes: monocytes from patients with early recurrence (R) used for ChIP-seq.

Article Snippet: Chromatin immunoprecipitation sequencing (ChIP-seq) libraries were constructed using SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina and SimpleChIP ChIP-seq Multiplex Oligos for Illumina (Cell Signaling Technology) according to the manufacturer’s instructions.

Techniques: Methylation, ChIP-sequencing